The NEBNext® Microbiome DNA Enrichment Kit facilitates enrichment of microbial DNA from samples containing methylated host DNA (including human), by selective binding and removal of the CpG-methylated host DNA. Importantly, microbial diversity remains intact after enrichment. Effective enrichment of microbial genomic DNA from samples contaminating host DNA Fast, simple protocol Enables microbiome whole genome sequencing, even for samples with high levels of host DNA Compatible with downstream applications including next generation sequencing on all platforms, qPCR and end point PCR Suitable for a wide range of sample types No requirement for live cells Optional protocol to retain separated host DNA Also effective for separation of organelle DNA (e.g. mitochondria, chloroplast) from eukaryote nuclear DNA
The NEBNext® Microbiome DNA Enrichment Kit facilitates enrichment of microbial DNA from samples containing methylated host DNA (including human), by selective binding and removal of the CpG-methylated host DNA. Importantly, microbial diversity remains intact after enrichment. Effective enrichment of microbial genomic DNA from samples contaminating host DNA Fast, simple protocol Enables microbiome whole genome sequencing, even for samples with high levels of host DNA Compatible with downstream applications including next generation sequencing on all platforms, qPCR and end point PCR Suitable for a wide range of sample types No requirement for live cells Optional protocol to retain separated host DNA Also effective for separation of organelle DNA (e.g. mitochondria, chloroplast) from eukaryote nuclear DNA