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HiScript II Reverse Transcriptase is a new reverse transcriptase obtained by in vitro molecular evolution technology on the basis of M-MLV (RNase H-) Reverse Transcriptase. Compared with the previous generation of HiScript Reverse Transcriptase, HiScript II Transcriptase has greatly improved thermal stability, with a half-life of more than 4 h at 50°C, and can remain stable for a long time at 55°C. It is suitable for reverse transcription of RNA templates with complex secondary structures for complex secondary structures. In addition, HiScript II Transcriptase adds multiple point mutations, which further enhances the template affinity and progression, which greatly improves the synthesis ability of full-length cDNA, and can obtain cDNA up to 20 kb. It has higher tolerance to common reverse transcription inhibitors and is very suitable for reverse transcription reaction of plant tissue RNA rich in polysaccharides and polyphenols.
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