qPCRBIO SyGreen 1-Step Go Lo-ROX

PCR Biosystems qPCRBIO SyGreen 1-Step Go Kit uses the latest developments in reverse transcriptase technology and buffer chemistry for efficient cDNA synthesis and PCR in a single tube. Our modified MMLV reverse transcriptase (RTase Go) is both thermostable and extremely active. The enzyme is blended with RNase inhibitor preventing degradation of RNA by contaminating RNase. The RTase is not inhibited by ribosomal and transfer RNAs, making total RNA an ideal substrate. PCR Biosystems SyGreen Mixes use an intercalating dye which does not inhibit PCR, unlike other popular dyes. qPCRBIO SyGreen 1-Step Mix uses antibodymediated hot start technology that prevents the formation of primer-dimers to improve reaction sensitivity and specificity. High-throughput screening has resulted in a buffer system that allows efficient amplification from GC-rich and AT-rich templates, under fast and standard cycling conditions.