IsoFast Bst Polymerase, 1600 units

IsoFast™ Bst Polymerase is a recombinant protein expressed in E. coli and represents the large fragment of Geobacillus stearothermophilus (formerly known as Bacillus stearothermophilus) DNA Polymerase. This portion of the protein maintains the protein 5’ to 3’ polymerase activity but lacks the 5’ to 3’ exonuclease activity.1 IsoFast™ Bst Polymerase displays strong strand displacement activity and is suitable for nucleic acid amplification methods such as whole genome amplification, multiple displacement amplification and isothermal amplification. We recommend a reaction temperature of 65ºC, however the enzyme works well over a broad temperature range, from 55ºC to 70ºC. It is heat inactivated at 80ºC. Designed for fast amplification speed, IsoFast™ Bst Polymerase gives rapid and consistent results across different target sequences and sample types. The enzyme is provided with a 2-part buffer system to ensure high yield and performance even under difficult conditions. Real-time detection with any qPCR thermocycler can be achieved by adding 20x Fluorescent Dye to the reaction. This is supplied separately, catalogue number PB80.30-2 and PB80.30-10.