5min Universal Ligation Mix is a ready-to-use 2 × premix solution, containing T4 DNA ligase that catalyzes the formation of a phosphodiester bond between adjacent 5'-phosphate and 3'-hydroxyl termini in double-strand DNA or RNA. This kit is suitable for sticky end ligation, blunt-end ligation and TA ligation. Buffer with optimized ligation enhancer makes the reaction more efficient and convenient. Ligation can be completed in 5 min at 25°C. The product can be used directly to transform many chemically competent cells.
5min Universal Ligation Mix is a ready-to-use 2 × premix solution, containing T4 DNA ligase that catalyzes the formation of a phosphodiester bond between adjacent 5'-phosphate and 3'-hydroxyl termini in double-strand DNA or RNA. This kit is suitable for sticky end ligation, blunt-end ligation and TA ligation. Buffer with optimized ligation enhancer makes the reaction more efficient and convenient. Ligation can be completed in 5 min at 25°C. The product can be used directly to transform many chemically competent cells.