Gibson Assembly Master Mix (NEB #M5510) has been reformulated with components containing Recombinant Albumin (rAlbumin) beginning with Lot #10229799. Learn more. Have you tried NEBuilder HiFi DNA Assembly? NEBuilder HiFi offers several advantages over NEB Gibson Assembly. For more information, visit NEBuilderHiFi.com. Gibson Assembly allows for successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility. Assembly and transformation in just under two hours Flexible sequence design (scar-less cloning) No PCR clean-up step required High transformation efficiencies for inserts up to 20 kb Easily adapted for multiple DNA manipulations, including site-directed mutagenesis
Gibson Assembly Master Mix (NEB #M5510) has been reformulated with components containing Recombinant Albumin (rAlbumin) beginning with Lot #10229799. Learn more. Have you tried NEBuilder HiFi DNA Assembly? NEBuilder HiFi offers several advantages over NEB Gibson Assembly. For more information, visit NEBuilderHiFi.com. Gibson Assembly allows for successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility. Assembly and transformation in just under two hours Flexible sequence design (scar-less cloning) No PCR clean-up step required High transformation efficiencies for inserts up to 20 kb Easily adapted for multiple DNA manipulations, including site-directed mutagenesis