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FastPure Plasmid Mini Kit purifies 1 - 5 ml of cultured bacteria and lyses the cells by an optimized SDS-alkaline method. The kit utilizes a silica-based membrane technology in the form of a convenient spin column, and binds plasmid DNA in a highly efficient manner under high-salt and low-pH conditions and largely remove proteins, genomic DNA, RNA, and other impurities. In the end, pure plasmid DNA is eluted from the silica membrane with a lowsalt, high-pH elution buffer. The kit recovers up to 35 µg of high-copy plasmid DNA per isolation procedure.The purified plasmid DNA is ready for use in biological experiments such as enzyme digestion, PCR, sequencing, ligation, transformation, and transfection of common passage cells.
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