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Expression of viral vectors in HEK 293 cells is a widely used procedure for obtaining sufficient quantities of a desired virus. The manufacturing and purification process of these products leaves the potential for impurities by host cell proteins (HCPs) from HEK 293 cells. The HEK 293 HCP ELISA Kit, 3G (F650S) can be used as a process development tool to monitor the removal of host cell impurities as well as in routine product release testing. The polyclonal antibodies used in this kit have been generated against and affinity-purified using a mild lysate of HEK 293 cells to obtain HCPs typically encountered in initial product recovery steps. The antibodies have broad coverage of ~90% to HEK 293 HCPs as determined by Antibody Affinity Extraction with 2D PAGE/Silver Stain and LC-MS. The kit provides the specificity and sensitivity (LOD ~0.35 ng/ml, LLOQ ~2 ng/ml) to detect HCPs with reproducibility that supports regulatory compliance. Please use Sample Diluent, Cat. No. I700, with these kits.
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